This menu contains various data-analysis features. These functions will be discussed in detail in the following sections.
Figure 1: Analysis Menu Options
GO-Slim is a reduced version of the Gene Ontology that contains a selected number of relevant nodes. The Run GO-Slim (online) function (under the Analysis → GO-Slim menu) generates a GO-Slim mapping for the available annotations. Different GO-Slims are available which are adapted to specific organisms. Blast2GO supports the following GO-Slim mappings: General, Plant, Yeast, GOA (GO-Association) and TAIR.
Use the Analysis > GO-Slim > Remove GO-Slim option to return to the original annotations.
Blast2GO provides EC annotation through the direct GO > EC mapping file available at the GO website. This means that only sequences with GO annotations will eventually show also EC numbers and that the GO annotation accuracy can be made extensive to Enzyme annotations.
To see the main Enzyme classes in the dataset it is possible to generate a distribution Enzyme Code chart on "charts" icon.
- Main Enzyme Classes: Shows the distribution of the 6 main enzyme classes over all sequences.
- Second Level Classes: Same as above but for the corresponding subclass.
Figure 3: Enzyme Code Distribution
Figure 4: Enzyme Code Statistic