Gene Ontology Mapping
Mapping is the process of retrieving GO terms associated to the Hits obtained by the BLAST search. Blast2GO performs four different mappings steps:
- BLAST result accessions are used to retrieve gene names or symbols making use of two mapping files provided by the NCBI (gene_info, gene2accession). Identified gene names are than searched in the species specific entries of the gene-product table of the GO database.
- GeneBank identifiers (gi), the primary blast Hit ids, are used to retrieve UniProt IDs making use of a mapping file from PIR (Non-redundant Reference Protein Database) including PSD, UniProt, Swiss-Prot, TrEMBL, RefSeq, GenPept and PDB.
- Accessions are searched directly in the dbxref table of the GO database.
- BLAST result accessions are searched directly in the gene-product table of the GO database.
Figure 1: Mapping options
405px2Run Mapping. Mapping will start.
Remove Mapping. Delete Mapping results for the selected sequences.
The mapping step needs protein ids to run. Make sure you ran blast against a protein database.
blastx - if one has nucleotide sequences
blastp - if one has protein sequences
Figure 5: Evidence Code Distribution of BLAST hits
Figure 6: Evidence Code Distribution for sequences
Figure 7: DB Resources of Mapping
Export Mapping Results
A tab separator text file can be exported with the corresponding mapping results (File > Export > Export Mapping Results).